SITC Archive Library

The Society for Immunotherapy of Cancer (SITC) hosted the "Workshop on Single Cell Techniques in Immunology and Cancer Immunotherapy" during the SITC 2017 Annual Meeting on November 9, 2017. Available here are the presentation slides and video from the workshop.

Program Organizers

• Gordon J. Freeman, PhD – Dana-Farber Cancer Institute
• Nir Hacohen, PhD – Massachusetts General Hospital
• Nicholas McGranahan, PhD – University College London

Program Summary

Historically, molecular analyses have been performed on heterogeneous populations of cells, where observed patterns represent a combination of the unique characteristics of each individual cell. In the last few years, single cell techniques combined with high-throughput technologies, such as multiplex quantitative PCR, next generation DNA and RNA sequencing, and state-of-the-art flow cytometry by time of flight (CyTOF), have revolutionized the field, allowing for high-dimensional analysis of isolated subpopulations of individual cells.

With these techniques, researchers have the opportunity to address key challenges barring advancements in the field of cancer immunotherapy, including resolving intratumoral heterogeneity, understanding rare tumor and immune cell populations, tracing cellular lineages and mutational rates, and elucidating complex cellular interactions within the tumor microenvironment both before and after treatment. To demonstrate the potential of single cell techniques in cancer immunotherapy research, this day-long workshop will focus on recent technical and analytical advances specific to single cell techniques to help drive the field forward.

Specifically, this program will begin with an overview of tumor heterogeneity and evolution under immune pressure, with a focus on neoantigen presentation, recognition, and evolution. In the second session of this workshop, changes in myeloid cell populations within the tumor microenvironment both prior to and after immunotherapy treatment will be presented. Moreover, predictors of response to therapy as well as the analysis of liquid tumor biopsies will also be discussed. In order to provide attendees guidance on how to apply single cell techniques to their own research, this workshop will conclude with a panel-led discussion with audience participation focused on experimental approaches and computational methods for single cell techniques.

Intended Audience

The intended audience for this annual, one-day program includes basic scientists and clinical investigators from academic institutions, industry and regulatory agencies. Additional audiences benefiting from this program include clinicians, graduate students, and postdoctoral fellows involved in cancer research and its translation into clinical practice.

Disclaimer: Due to the rapidly-evolving field of cancer immunotherapy, some of the content on this page may be out of date. For current information from SITC on cancer immunotherapy, scroll down to view Related Entries and Links.

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