SITC Archive Library

NanoString Technologies hosted "Quantifying T-cell receptor diversity via expression analysis," a sponsored symposia during the 2021 SITC Annual Meeting. Available here is the recording from the symposia.

Program Summary

T-cell receptor (TCR) gene segments are comprised of numerous discontinuous coding segments that somatically rearrange to form the TCR gene during the development of a T cell. The TCR repertoire is highly diverse and estimated to exceed 106 sequences in humans. Clonal expansion of T cell populations can be observed in physiological T-cell responses and in pathology, e.g., T-cell lymphoma, and are characterised by a loss of TCR clonality and emergence of dominant TCR clonotypes. Assessment of T-cell clonality as a biomarker of T-cell malignancy or an inflammatory response can be challenging. By directly quantifying the expression of each individual TCR variable region, the NanoString nCounter® Analysis System can provide insight on TCR diversity without sequence information of the clonotypes. In this workshop, attendees learned about the new nCounter TCR Diversity Gene Expression Panel as well as a case study on the use of the panel for clinical research.

Sponsored Symposia content and views expressed therein are those of the sponsoring organization and not of SITC.