I am Associate Professor and Director of the Multiplex Immunofluorescence (IF) and Digital Pathology Laboratory in the Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center. I am a pathologist with strong background in surgical pathology and research, with main interest on elucidating the molecular mechanism involved in the pathogenesis and progression of lung cancer and other tumors, including both human tissues and mouse models by applying histopathology, immunohistochemistry (IHC), multiplex IF, Multiplex RNAscope, histomorphometry and digital analysis using different scanning system as Aperio AT2, Aperio IF, MultiOmixTM, Mantra and Vectra®-Polaris and image analysis software’s as Image Pro-plus, Aperio Tool Box, GENIE, InForm, HALLO, Tissue Studio and Image Mainer. I am particularly interested in the characterization of immune response in lung cancer and the development of novel biomarkers for immunotherapy. I have experience in IHC, multiplex mIF, multiplex RNAscope, western blot, DNA, RNA extraction (PCR), confocal, and digital pathology analysis that I acquire during my doctoral and my postdoctoral fellows in Brazil and USA. I have over 10 years of experience in research, teaching technicians, graduate students, doctors and postdoctoral fellows. I also have extensive experience in performing standard laboratory techniques for molecular biology such cell culture, manual and automated IHC and mIF staining and experimental animal models. I am author and co-author of more than 100 paper in lung cancer, interstitial lung diseases from human and animal models, and immunoprofiling using different image analysis-based assessment; several abstracts and presentations at national and international scientific meetings, three book chapters and many other reports on pulmonary pathology and medicine topics. I am highly training to develop and optimize immunoprofiling panels by IHC, multiplex IF and RNAscope, for immune markers to develop prognostic immune profiling for Non-Small Cell Lung Carcinoma and other types of cancer. In my position, I supervise the laboratory of multiplex IF and image analysis, including RNAscope and Mass spectrometry (CyTOFF and MIBI) techniques. I have under my supervision postdoctoral fellows, research investigators and research scientist pathologists as well as highly specialized research assistants carefully trained on IHC, multiplex IF, multiplex RNAscope and digital scanning techniques. My roles include laboratory personnel and pathologist supervision, project coordination, pathology IHC and multiplex IF quality control. Setup new multiplex techniques including antibodies validation and optimization to incorporate and expand the arsenal of new tissue analysis techniques and protocols that can be included in our current multiplex IF work.